LOCUS       21-02_pDV74.20-C        6439 bp    DNA     circular     23-JUL-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12914868|
COMMENT     VNTDBDATE|-12915076|
COMMENT     LSOWNER|
COMMENT     VNTNAME|21-02_pDV74.20-CL7-NT-1_Template_Vector|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     promoter        157..173
                     /vntifkey="30"
                     /label=T7_P
                     /note="T7 RNA polymerase promoter"
     terminator      1541..1587
                     /vntifkey="43"
                     /label=T7_TERM
                     /note="T7 RNA Pol Terminator"
     CDS             complement(5130..6209)
                     /vntifkey="4"
                     /label=LACI
                     /note="lac I coding sequence"
     CDS             complement(2175..2987)
                     /vntifkey="4"
                     /label=KN(R)
                     /note="Kanamycin resistance gene"
     rep_origin      1624..2079
                     /vntifkey="33"
                     /label=F1_ORI
                     /note="f1 origin of replication"
     rep_origin      3696..3696
                     /vntifkey="33"
                     /label=PBR322_ORI
                     /note="pBR322 origin of replication"
     misc_signal     244..570
                     /vntifkey="87"
                     /label=Thioredoxin\(Trx)
     misc_structure  589..963
                     /vntifkey="88"
                     /label=CL7\Affinity\Tag
     misc_signal     1099..1395
                     /vntifkey="87"
                     /label=SUMO
     misc_signal     964..1095
                     /vntifkey="87"
                     /label=LNK1\\(44\aa)
     misc_feature    244..1404
                     /vntifkey="21"
                     /label=N-Terminal\Tag\(Trx-CL7-2x[PSC]-His8-SUMO)\[MW\42.9\kDa]
     misc_feature    985..1008
                     /vntifkey="21"
                     /label=[PSC-1]
     misc_feature    1033..1056
                     /vntifkey="21"
                     /label=[PSC-2]
     misc_feature    1060..1080
                     /vntifkey="21"
                     /label=<<<\His8\>>>
     misc_feature    1426..1455
                     /vntifkey="21"
                     /label=!!!!!!!!!!!\(KpnI/XhoI)\Restriction\Fragment\(47\bps)\\!!!!!!!!!!!!!!!!!!!
     misc_feature    1396..1404
                     /vntifkey="21"
                     /label=LNK2\(3\aa)
     misc_feature    175..199
                     /vntifkey="21"
                     /label=LAC\Operator
BASE COUNT     1589 a      1679 c      1641 g      1530 t 
ORIGIN
        1 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg 
       61 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt 
      121 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat 
      181 tgtgagcgga taacaattcc cctctagaaa taattttgtt taaccttaag aaggagatat 
      241 accatgagcg ataaaattat tcacctgact gacgacagtt ttgacacgga tgtactcaaa 
      301 gcggacgggg cgatcctcgt cgatttctgg gcagagtggt gtggtccgtg caaaatgatc 
      361 gccccgattc tggatgaaat cgctgacgaa tattctggca aactgaccgt tgcaaaactg 
      421 aacatcgatc aaaaccctgg cactgcgccg aaatatggca tccgtggtat cccgactctg 
      481 ctgctgttca aaaacggtga agtggcggca accaaagtgg gtgcactgtc taaaggtcag 
      541 ttgaaagagt tcctcgacgc taacctggca ggtagtggta cgaattctga accgggtgaa 
      601 gcaactggtg agggtgaacc agttaacaac aaatggctgg ataacgcagg taaagatctg 
      661 ggttctccag ttcctgatcg cattgctgat aagctgcgcg atcaagaatt tgaatccttt 
      721 gacgatttcc gtgaaacctt ttgggaagaa gtctctaaag acccggaact gagcaaacag 
      781 ttctcccgca acaacaacga ccgcatgaaa gtgggcaaag ctccgaaaac ccgtactcaa 
      841 gacgtttctg gcaaacgcac tagctttgaa ctgaaccatg agaaaccgat tgaagaaaac 
      901 ggcggtgttt atgatatgga caacatctcc gtggttacgc cgaaacgtaa catcgatatc 
      961 gaaaaccatg gtgacaatac gtctctggaa gtactgtttc aaggtcctag ttctaacacc 
     1021 tcttctaacg gcttagaggt tctgtttcag ggtccaggtc atcatcacca tcaccatcat 
     1081 cacaacgaga cgtctggttc tctgcaagat tctgaagtta atcaggaagc taaaccggaa 
     1141 gtaaaaccag aggttaaacc agaaactcac attaacctga aagtgagcga tggttcttcc 
     1201 gaaatctttt tcaagattaa aaagactact ccgctgcgtc gtctgatgga agcattcgcg 
     1261 aaacgccaag gtaaagaaat ggattctctg cgctttctgt atgacggcat tcgcattcag 
     1321 gctgaccaag cacctgagga tctggacatg gaagacaatg acattatcga agctcatcgt 
     1381 gaacagattg gtggttctgg taccctgcct gaaactggtt ctagcgaagc ttctagcaat 
     1441 gattaatgac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga 
     1501 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc 
     1561 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat 
     1621 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     1681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     1741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     1801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     1861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     1921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     1981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     2041 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc aggtggcact tttcggggaa 
     2101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     2161 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca 
     2221 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg 
     2281 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca 
     2341 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca 
     2401 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt 
     2461 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc 
     2521 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa 
     2581 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct 
     2641 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt 
     2701 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc 
     2761 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca 
     2821 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct 
     2881 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa 
     2941 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta 
     3001 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt 
     3061 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     3121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     3181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     3241 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     3301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     3361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     3421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     3481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     3541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     3601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     3661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     3721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     3781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     3841 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct 
     3901 ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc 
     3961 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg 
     4021 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 
     4081 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 
     4141 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg 
     4201 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta 
     4261 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg 
     4321 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga 
     4381 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg 
     4441 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc 
     4501 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc 
     4561 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac 
     4621 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc 
     4681 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc 
     4741 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg 
     4801 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag 
     4861 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc 
     4921 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca 
     4981 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg 
     5041 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga 
     5101 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     5161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc 
     5221 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 
     5281 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 
     5341 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact 
     5401 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc 
     5461 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc 
     5521 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga 
     5581 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa 
     5641 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg 
     5701 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag 
     5761 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg 
     5821 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc 
     5881 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc 
     5941 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga 
     6001 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg 
     6061 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa 
     6121 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct 
     6181 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg 
     6241 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg 
     6301 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag 
     6361 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac 
     6421 ggggcctgcc accataccc 
//
